Automated workflow for scATAC-seq analysis, covering QC, clustering, motif activity, and peak linkage.

Install

mkdir -p .claude/skills/omics-scatac && curl -L -o skill.zip "https://agentskills.codes/api/skills/download/14419" && unzip -o skill.zip -d .claude/skills/omics-scatac && rm skill.zip

Installs to .claude/skills/omics-scatac

Activation

This is the description your AI agent reads to decide when to run this skill — the better it matches your request, the more reliably it fires.

Single-cell ATAC-seq — fragment import, ATAC QC (TSS / fragment-size / FRiP / doublets), feature matrix (tiles / MACS3 peaks), spectral (LSI) clustering, motif activity (chromVAR), gene activity, peak–gene linkage, scRNA label transfer.
236 charsno explicit “when” trigger
Advanced

Key capabilities

  • Import fragments into cell×feature matrix
  • Perform ATAC QC (TSS, fragment size, FRiP, doublets)
  • Generate feature matrices (tiles/peaks)
  • Call peaks using MACS3 per cluster
  • Perform spectral (LSI) embedding and Leiden clustering
  • Calculate gene activity scores

How it works

The skill processes single-cell ATAC-seq data by importing fragments, performing quality control, and generating feature matrices. It then applies spectral embedding for clustering and calculates gene activity scores.

Inputs & outputs

You give it
Fragments file, AnnData object, study description
You get back
Cell×feature matrix, QC report, peak calls, clustered data, gene activity scores

When to use omics-scatac

  • Importing and QC-ing fragment files
  • Calling peaks with MACS3
  • Analyzing gene activity and peak linkages

About omics-scatac

This skill manages single-cell ATAC-seq pipelines, including fragment import, QC, and peak calling. It automates spectral clustering, chromVAR motif analysis, and scRNA label transfer.

Single-cell ATAC-seq — fragment import, ATAC QC (TSS / fragment-size / FRiP / doublets), feature matrix (tiles / MACS3 peaks), spectral (LSI) clustering, motif activity (chromVAR), gene activity, peak–gene linkage, scRNA label transfer.

When not to use it

  • When inferring GRN from accessibility alone
  • When using PCA instead of spectral embedding for ATAC data
  • When expecting gene activity scores to be measured expression

Prerequisites

omics_preflight(modality="scatac") passesA fragments file (`fragments.tsv.gz` + `.tbi`)A `summarize` report + free-text study description

Limitations

  • GRN inference is not a pure-scATAC step
  • Accessibility ≠ expression
  • Distance ≠ regulation

How it compares

This skill provides a structured workflow for scATAC-seq analysis, including specialized QC and embedding methods for sparse ATAC data, which differs from generic single-cell analysis pipelines.

Compared to similar skills

omics-scatac side by side with the closest alternatives in the catalog.

SkillInstallsUpdatedSafetyDifficulty
omics-scatac (this skill)01moNo flagsAdvanced
quant-analyst1032moNo flagsAdvanced
umap-learn62moReviewIntermediate
embedding-strategies82moNo flagsIntermediate

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Example prompts that trigger this skill in your AI assistant.

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